Full Western blot protocol, including reagent preparation, sample preparation, Bradford assay/standard curve, gel preparation, SDS-PAGE, transfer, blocking, antibody incubation, and analysis using ImageJ.


Pipetting learning module.


Full Genotyping protocol.


Step-by-step video on how to make solutions in the laboratory.


Microscopy and Image Analysis learning module divided into three parts.

MICROSCOPY AND IMAGE ANALYSIS

PT.1    Pt.2    Pt.3


Image Processing and Quantitative Image Analysis learning module using ImageJ, divided into three parts.

IMAGE PROCESSING

Pt.1    Pt.2    Pt.3


Manual on how to use Photoshop to process images from microscopes.


Good practices and guidelines for image acquisition with microscopes. 


Introductory video on the NIS Elements software, used to control our two-photon microscope.


Guide on immunohistochemical staining methods.

Immunofluorescent Staining for Free-Floating Slice


List of equipment available at Indiana University Bloomington.


Guide to conducting perfusion on mice. Credit Caliel Hines.